EKSPRESI IMUNOSITOKIMIA CALRETININ PADA DIAGNOSIS SITOLOGI EFUSI PLEURA GANAS DENGAN GAMBARAN SITOMORFOLOGI CURIGAADENOKARSINOMA PARU
Keywords: calretinin, cytology effusion
AbstractMore than 75% of people with a malignant pleural effusion have lymphoma or cancers of the breast, lung, ovary or malignant mesothelioma. Effusion aspiration can reduce invasive procedure, however by morphologic criteria alone may be difficult to distinguish malignant cell. Calretinin is a calciumbinding protein that is expressed normally in neurons of the central and peripheral nervous system, and in the mesothelial cell. The aim of this study was to prove calretinin expression in malignant effusion originate from malignant mesothelioma. Descriptive analytic study using cross sectional design. Twenty paraffin embedded cells of pleural effusion that meet the criteria were stained with calretinin. Positive staining was defined as nuclear and cytoplasmic staining with intensity weak or strong. The immunoreactivity was evaluated blindly by two pathologists. Calretinin staining was negative in 6 samples, positive 1 (weak) in 8 samples, positive 2 (moderate ) in 2 samples and positive 3 (strong) in 4 samples, with strong intensity in 6 sampes and weak in 8 samples. There was strong positive and strong intensity calretinin expression in malignant effusionoriginates from malignant mesothelioma.